Blastema cell cycle in vitro and attempted restimulation of blastema cell cycling in denervated blastemata of the adult newt,Notophthalmus viridescens
✍ Scribed by Tomlinson, Bruce L. ;Globus, Morton ;Vethamany-Globus, Swani
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 805 KB
- Volume
- 232
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
The durations of the adult newt blastema cell-cycle phases were determined in vitro. In both ganglionated (G) and nonganglionated (nG) blastemata the total cell-cycle time was 62 hr; GI + %M, S, and G2 + YzM were 18, 37, and 7 hr, respectively. The lack of a significant difference in the durations of cell-cycle phases between G and nG explants suggests that nerve withdrawal does not influence the rate of progression through the cycle. A novel strategy was employed involving cell-cycle suppression (denervation in vivo) and continuous labeling followed by restimulation with nerves in vitro, in anticipation that nerve-dependent restricted cells will resume cycling. The data indicate that following prolonged denervation (48 hr), blastema cells may become refractory to neurotrophic factor.