Bladder tissue formation from cultured bladder urothelium
โ Scribed by Siam Oottamasathien; Karin Williams; Omar E. Franco; John C. Thomas; Katrina Saba; Neil A. Bhowmick; Andrea Staack; Romano T. Demarco; John W. Brock III; Simon W. Hayward; John C. Pope IV
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 268 KB
- Volume
- 235
- Category
- Article
- ISSN
- 1058-8388
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Trypsinization (WT) was employed to disaggregate urothelial cells from normal human urinary bladder mucosa for the preparation of primary cultures. Urinary bladders of two male adults both 25 years old were obtained autopsy 1-2 h after death. The mucosa was incubated in HBSS containing 0.25% trypsin