Bisulfite Sequencing Protocol Displays both 5-Methylcytosine and N4-Methylcytosine

✍ Scribed by Giedrius Vilkaitis; Saulius Klimašauskas

• Book ID: 102559076
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 91 KB
• Volume: 271
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1999.4116

✦ Synopsis

binant adenoviral vector expressing the wild-type FHIT gene. The new methodology we developed for assay of FHIT Ap 3 A hydrolase activity by TLC fluorescent image analysis offers the following advantages over conventional assay methods: (i) no radioactive materials involved; (ii) high sensitivity of fluorescent analysis; (iii) rapid and simultaneous TLC fluorescent imaging and quantitative reporting; (iv) low cost compared to the custom radioactive labeling of substrate; and (v) could be applied for assaying activities of other Ap n A hydrolases in the cells.

Acknowledgments.