๐”– Bobbio Scriptorium
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Biotinylated peptides/proteins: II. Identification of biotinylated lysyl phenylthiohydantoins

โœ Scribed by John S. Smith; Brian T. Miller; Alexander Kurosky


Book ID
107718964
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
399 KB
Volume
197
Category
Article
ISSN
0003-2697

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๐Ÿ“œ SIMILAR VOLUMES


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Recent findings have indicated that certain hydroxyl groups of peptides have enhanced intrinsic chemical reactivity and can be O-acylated by N-hydroxysuccinimide esters of biotin. Analytical procedures are described for the identification of O-acylated derivatives of seryl, threonyl, and tyrosyl res

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Site-specific, enzymatic biotinylation of recombinant proteins can be exploited to circumvent many problems associated with the use of biotinylating reagents in vitro and to overcome some of their inherent limitations. Additionally, biotinyl proteins can be purified to near-homogeneity in a single s