Biotinylated peptides/proteins: II. Identification of biotinylated lysyl phenylthiohydantoins
โ Scribed by John S. Smith; Brian T. Miller; Alexander Kurosky
- Book ID
- 107718964
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 399 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Recent findings have indicated that certain hydroxyl groups of peptides have enhanced intrinsic chemical reactivity and can be O-acylated by N-hydroxysuccinimide esters of biotin. Analytical procedures are described for the identification of O-acylated derivatives of seryl, threonyl, and tyrosyl res
Site-specific, enzymatic biotinylation of recombinant proteins can be exploited to circumvent many problems associated with the use of biotinylating reagents in vitro and to overcome some of their inherent limitations. Additionally, biotinyl proteins can be purified to near-homogeneity in a single s