Biosynthetic preparation of the tritium labelled 3α-hydroxy-5α-pregnan-20-one, an allosteric modulator of gabaergic neurotransmission
✍ Scribed by Astrid Jussofie; Ibrahim Jussofie; Martin Stoppok; E. Rodney Lax
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- French
- Weight
- 152 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
A procedure for the biosynthetic preparation of 3α‐hydroxy‐5α‐[1,2,6,7‐^3^H]‐pregnan‐20‐one (^3^H‐HPO) with high specific activity convenient for binding studies was developed. ^3^H‐HPO was synthesized from [1,2,6,7‐^3^H]progesterone using a microsomal preparation from rat liver and NADPH. The progesterone metabolite was purified by silica gel 60 and Sephadex LH 20 chromatography. The identity of the product was confirmed by reverse isotopic dilution of chemically synthesized and highly purified 3α‐hydroxy‐5α‐pregnan‐20‐one. The yield for radiolabelled HPO was 60% and its purity was > 98%.