A method for obtaining highly purified NADH in a dry, solid, and stable form is described. The method involves improvements of the ion-exchange and reversed-phase chromatographic procedures of C. J. Newton and S. M. Faynor, and D. B. Northrop (Anal. Biochem., 1983, 132, 50-53). The necessary time to
Biosynthetic Preparation of Isotopically Labeled Heme
β Scribed by M. Rivera; F.A. Walker
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 685 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0003-2697
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Visible resonance Raman spectra in the low-frequency region (200-500 cm-') are reported for hemoglobin ( H b ) reconstituted with heme that is deuterated at the meso carbon atoms (meso-d,). Spectra were obtained in the deoxy form and in the immediate photoproduct of the carbonmonoxide adduct, HbCO.
## Abstract ^11^Cβlabelled galactose, glycerol and mannitol have been prepared biosynthetically from ^11^Cβlabelled carbon dioxide using species of marine algae. Following biosynthesis the labelled compounds are rapidly extracted from the algae and purified by liquid chromatography.
## Abstract Retinyl acetate (4a) has been prepared with a tritium label at the Cβ11 and Cβ12 positions by partial reduction of oxenin (1) with tritium gas followed by acetylation and rearrangement. Specific activities of up to 40 curies/mmole have been attained. By alkaline hydrolysis, retinol (5)