Biosynthetic Origins of the Carbon Skeleton of Heme d1

✍ Scribed by J.C. Matthews; R. Timkovich

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 483 KB
• Volume: 21
• Category: Article
• ISSN: 0045-2068
• DOI: 10.1006/bioo.1993.1009

✦ Synopsis

({ }^{13})-labeling experiments were performed to determine the simple precursor metabolites that contribute to the tetrapyrrole skeleton of heme (\mathrm{d}{1}). By use of (5-\left[4^{-13} \mathrm{C}\right]) aminolevulinic acid it was determined that the skeleton is derived from (8 \mathrm{eq}) of this acid and that the labeled carbons appeared at sites (\mathrm{C1}, \mathrm{C} 3, \mathrm{C} 6, \mathrm{C} 8, \mathrm{C11}, \mathrm{C13}, \mathrm{C17}), and (\mathrm{C} 19) (IUB-IUPAC tetrapyrrole nomenclature). In the course of this study, it was discovered that the glycine-succinyl-coenzyme A pathway to 5-aminolevulinate is not operative in Pseudomonas aeruginosa. ({ }^{13} \mathrm{C}) chemical shifts are reported for the complete skeleton of the free base porphyrin (\mathrm{d}{1}) methyl ester derivative, except for C4 and C9. 1993 Academic Press. Inc.