Biosynthesis regulation of theβ-glucosidase produced by a yeast strain transformed by genetic engineering

✍ Scribed by M. Leclerc; P. Chemardin; A. Arnaud; R. Ratomahenina; P. Galzy; C. Gerbaud; A. Raynal

• Book ID: 104758684
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 276 KB
• Volume: 146
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00402336

✦ Synopsis

The biosynthesis of the beta-glucosidase enzyme was studied in a transformed yeast obtained by cloning in Saccharomyces cerevisiae the structural gene coding for beta-glucosidase in Kluyveromyces fragilis. The enzyme biosynthesis was found to be non-adaptative, and repressed by glucose. These features are similar to those observed in K. fragilis. beta-Glucosidase activity in the transformed yeast was much higher than in K. fragilis. We attempted to ferment cellobiose with the transformed yeast: practically no cellobiose was consumed, growth and ethanol production were negligible. Warburg experiments showed that cellobiose fermentation did not occur when the respiratory chain was not functioning.