Biosynthesis of the yeast cell wall: Selective assays and regulation of some mannosyl transferase activities
✍ Scribed by M. Victoria Elorza; G. Larriba; J. R. Villanueva; R. Sentandreu
- Publisher
- Springer Netherlands
- Year
- 1977
- Tongue
- English
- Weight
- 665 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0003-6072
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✦ Synopsis
Biosynthesis of the yeast cell wall: Selective assays and regulation of some mannosyl transferase activities. Antonie van Leeuwenhoek 43: 129-142.
Assays have been developed for some transfer reactions involved in the synthesis ofSaccharomyces cerevisiae wall mannoproteins, both in a particulate preparation in the presence of EDTA or Triton X-100, and after lipid extraction with chloroform-methanol at -20 C.
The mannosyl transferase activities were also studied in cells made permeable to GDP-mannose by toluene-ethanol treatment ("in situ"). In these permeabilized cells, the glycosylating reactions dependent on lipid carriers (dolichol derivatives) did not function, but those independent of them were unaffected.
The lipid-independent mannosyl transferase activities were partially inhibited by nucleotide diphosphates probably in a competitive manner. Increase of the nucleotide diphosphate pool "in vivo" might slow down the speed of the transfer reactions carried out by the mannan synthetase system.