Biosynthesis of the polysialic acid capsule inEscherichia coliK1
β Scribed by E Vimr; S Steenbergen; M Cieslewicz
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 1020 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1476-5535
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Transposon and deletion analysis of the cloned K1 capsule biosynthesis genes of Escherichia coli revealed that approximately 17 kb of DNA, split into three functional regions, is required for capsule production. One block (region 1) is required for translocation of polysaccharide to the cell surface
The incorporation of radioactive N-acetylglucosamine into murein and lipopolysaccharide of synchronized cells ofEscherichia coli K 12 was followed over 100 min in the presence of antibiotics. At 20 min intervals cell walls were prepared. Lipopolysaccharide and murein sacculi were isolated and the ra