Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues
✍ Scribed by Kent Kirshenbaum; Isaac S. Carrico; David A. Tirrell
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 85 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1439-4227
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## Abstract __The unsaturated amino acid 2‐amino‐3‐methyl‐4‐pentenoic acid (__E__‐Ile) was prepared in the form of its (2__S__,3__S__),(2__R__,3__R__) and (2__S__,3__R__),(2__R__,3__S__) stereoisomeric pairs. The translational activities of__ SS__‐__E__‐Ile and__ SR__‐__E__‐Ile were assessed in an_
## Abstract An __Escherichia coli__ suppressor tRNA^Phe^ (tRNA^Phe^~CUA~) was misacylated with 4‐iodo‐L‐phenylalanine by using the A294G phenylalanyl–tRNA synthetase mutant (G294‐PheRS) from __E. coli__ at a high magnesium‐ion concentration. The preacylated tRNA was added to an __E. coli__ cell‐fre