Biosynthesis of labeled fumonisins in liquid cultures ofFusarium moniliforme

✍ Scribed by Ronald D. Plattner; Darcy D. Shackelford

• Book ID: 104787173
• Publisher: Springer Netherlands
• Year: 1992
• Tongue: English
• Weight: 416 KB
• Volume: 117
• Category: Article
• ISSN: 0301-486X
• DOI: 10.1007/bf00497274

✦ Synopsis

Fumonisins were readily produced in cultures of Fusarium moniliforme using a defined liquid medium. Addition of 200 mg of d3-methyl L-methionine to 100-ml cultures of F. moniliforme gave increased overall yields and high levels of deuterium (2H) incorporation into fumonisin B1. Approximately 90% of the resulting fumonisin B1 contained 6 deuterium atoms, while 9% of the product contained 3 deuterium atoms. Deuterium was shown to be incorporated exclusively in the methyl groups of the fumonisin backbone. The addition of as little as 5 mg of labeled methionine stimulated fumonisin production, but only about 5% of the fumonisin produced contained 3 deuterium atoms.