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Biosynthesis of Gramicidins and Tyrocidines in Cell-Free Preparations from Bacillus brevis *

✍ Scribed by Uemura, Isamu; Okuda, Kiyoshi; Winnick, Theodore


Book ID
126123670
Publisher
American Chemical Society
Year
1963
Tongue
English
Weight
894 KB
Volume
2
Category
Article
ISSN
0006-2960

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Cloning of the tyrocidine synthetase 1 g
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The entire structural gene for tyrocidine synthetase 1 from Bacillus brevis ATCC 8185 has been cloned and expressed in Escherichia coli. Transformed E. coli cells were screened for their ability to produce tyrocidine synthetase 1 by in situ immunoassay using antibodies against gramicidin S synthetas