Biosynthesis of acaterin: Metabolic fate of sn-3 hydrogens of glycerol during the formation of 4-dehydroacaterin

✍ Scribed by Yasuyo Sekiyama; Yoshinori Fujimoto; Keiji Hasumi; Akira Endo

• Publisher: Elsevier Science
• Year: 1999
• Tongue: French
• Weight: 197 KB
• Volume: 40
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(99)00681-4

✦ Synopsis

The metabolic fate of the pro-R and Wo-S hydrogens at the sn-C-3 position of glycerol during the formation of 4-dehydt _~__L~n has been investigated using Pseudomonas sp. A92. Feeding studies of sn-(3R)-and sn-(3S)-(3-2H)glyccruls revealed that 5E hydrogen of 4-d~ydroaca~n originates mainly from pro-R hydrogen at sn-C-3 of glycerol, while 5Z hydrogen comes mainly from pro-8. These results ruled out pyruvate and lactate as the immediate precursor of the.C 3 branched unit of acaterin, and suggest 1,3-bisphosphoglyceric acid or its biological equivalent as the precursor.