𝔖 Bobbio Scriptorium
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Biosensors for the measurement of toxicity of wastewaters to activated sludge†

✍ Scribed by Evans, Michael R.; Jordinson, Greg M.; Rawson, David M.; Rogerson, Jonathan G.

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
124 KB
Volume
54
Category
Article
ISSN
1526-498X

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✦ Synopsis

When wastewaters from chemical manufacturing are discharged for biological treatment it is important to ensure that the presence of potentially toxic chemicals is below concentrations that may impair the performance of the treatment plant. This report describes the use of mediated amperometric biosensors incorporating activated sludge to provide rapid determination of toxicity in wastewaters to activated-sludge-based water-treatment plants. Redoxmediated interrogation of the microbial consortium comprising the biosensor biocatalyst allowed real-time monitoring of metabolic activity and the detection of perturbation due to toxic challenge. A time-independent inhibition value was determined within 30 min of exposure, using a mathematical model based on a hyperbolic relationship between exposure time and biosensor inhibition. Comparative testing of the biosensors and an activated sludge respiration inhibition test (ASRIT) against 35 wastewater samples, from a wide variety of industrial discharges, showed the close correlation coefficient of 0É924 between the two test methods.

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