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Biosensor system for continuous flow determination of enzyme activities. I. Determination of glucose oxidase and lactic dehydrogenase activities

✍ Scribed by Hirokazu Okuma; Shuichi Sekimukai; Masakazu Hoshi; Kenzo Toyama; Etsuo Watanabe


Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
438 KB
Volume
11
Category
Article
ISSN
0141-0229

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A sensitive flow system for electrochemical determination of the reduced coenzyme NADH was developed using immobilized NADH oxidase from Buciflus lichenifomis. The immobilized enzyme was packed into a 20 mm x 2 mm I.D. mini-column, and the sample volume used was only 5 ~1 per assay, with an assay t