Bioreactor for glycerol conversion into H2 by bacterium Enterobacter aerogenes

✍ Scribed by Sergei A. Markov; Jared Averitt; Barbara Waldron

• Book ID: 103832818
• Publisher: Elsevier Science
• Year: 2011
• Tongue: English
• Weight: 223 KB
• Volume: 36
• Category: Article
• ISSN: 0360-3199
• DOI: 10.1016/j.ijhydene.2010.09.090

✦ Synopsis

Glycerol was used as a substrate for H 2 production by bacterium Enterobacter aerogenes in the test tubes and bioreactor. A BioFlo/CelliGen 115 bioreactor (10 L working volume) was utilized to conduct the experiments for conversion of glycerol into H 2 by E. aerogenes cells.

The highest H 2 production rate was observed under 2% glycerol in the culture medium. The glycerol uptake efficiency by bacteria in the bioreactor was found to be 65% during the 6 day period, matching glycerol uptake efficiency observed in the test tubes experiment (65%).Hydrogen production from glycerol (2% glycerol, v/v) by E. aerogenes in the bioreactor and test tubes was measured over the 6 days, showing the maximal H 2 rate at 650 mL g À1 dry weight h À1 . The yield of H 2 production from glycerol at 0.89 mol/mol in the bioreactor was high, corresponding to the theoretical yield of 1 mol of H 2 per 1 mol of glycerol.