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Biophysical properties of human astrocytic brain tumor cells in cell culture

✍ Scribed by Peter McL. Black; Paul L. Kornblith

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
542 KB
Volume
105
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

For malignant cells cultured from a human astrocytoma, electrophysiological characteristics of the plasma membrane included specific resistivity of 446.82 ± 279.5 ohm·cm^2^, specific capacitance of 0.758 ± 0.52 microfarads/cm^2^, time constant 0.318± 0.10 msec. The resting membrane potential averaged‐14.07 ± 7.4 mV; the mean input resistance 8.1 ± 4.0 megohms. The average cell area was 1638 ± 585 ±^2^ for contactual and 1919 ± 989 ±^2^ for noncontactual cells. Changes in input resistance and resting membrane potential were observed with increasing time in culture, possibly reflecting cell cycling. There did not appear to be electrical coupling in this cell line.

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