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Biophysical Characterization of the Igg Binding Domains of Protein a In Staphylococcus Aureus

✍ Scribed by Hagarman, Andrew; Franch, William R.; Oas, Terrence G.


Book ID
122272749
Publisher
Biophysical Society
Year
2011
Tongue
English
Weight
35 KB
Volume
100
Category
Article
ISSN
0006-3495

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One, two or four IgG-binding domains of the Staphylococcus aureus Protein A (SPA) were inserted into the LamB protein which was expressed under control of the tac promoter. The chimeric proteins were shown to be exposed at the cell surface by analysis of isolated outer membranes and also by testing