Bioorganic Mechanisms of the Formation of Free Radicals Catalyzed by Glucose Oxidase

✍ Scribed by Svetlana Trivić; Vladimir Leskovac; Jasmina Zeremski; Miroslav Vrvić; Gary W. Winston

• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 82 KB
• Volume: 30
• Category: Article
• ISSN: 0045-2068
• DOI: 10.1006/bioo.2001.1229

✦ Synopsis

In this communication, we have described the activation of several xenobiotics by glucose oxidase from Aspergillus niger. The following compounds are readily reduced by D-glucose, in the presence of glucose oxidase: p-nitroso-N,N-dimethylaniline, methyl-1,4-benzoquinone, and 7,7,8,8-tetracyano-quinodimethane. In each case, the products of enzymatic reduction undergo a dismutation reaction with the parent compound and thus afford the formation of free radicals. In some cases, and at an appropriate pH value, the transformation of a parent compound into free radicals is almost quantitative. Under optimal conditions, free radicals are stable for several minutes in aqueous solutions under physiological conditions. ᭧ 2002 Elsevier Science (USA)