## Abstract Fibronectin, a plasma protein immunologically identical with a major surface protein of normal fibroblasts, was found to bind to collagen and gelatin. A solid phase enzyme immunoassay was used for the binding tests. Collagen, gelatin or various control proteins were adsorbed to a plasti
Biomplant surfaces: Binding of fibronectin and fibroblast adhesion
β Scribed by Mario Cannas; Fernando Denicolai; Lawrence X. Webb; Dr. Anthony G. Gristina
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 537 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0736-0266
No coin nor oath required. For personal study only.
β¦ Synopsis
The adhesion of baby hamster kidney 21C/13 fibroblasts to surfaces of passivated titanium, carbon fibers, bioactive glasses B5 and B6, fibronectin-precoated passivated titanium, and fibronectin-precoated B6 was quantified. The order of adhesive cell avidity for the uncoated surfaces was passivated titanium (greatest), B6 and carbon fibers (intermediate), and B5 (least). Precoating with fibronectin enhanced the adhesive characteristics of fibroblasts on passivated titanium and B6 by 74% and 118%, respectively.
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## Abstract Integrinβmediated cell adhesion to proteins adsorbed onto synthetic surfaces anchors cells and triggers signals that direct cell function. In the case of fibronectin (Fn), adsorption onto substrates of varying properties alters its conformation/structure and its ability to support cell
## Abstract Multilayered fibroblast sheets have applications as cell transplants for tissue engineering. One way to increase their therapeutic efficacy is to increase cell numbers in a graft, but the factors influencing multilayered growth remain poorly understood. In this study, we investigated th