𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells

✍ Scribed by Sabia Taqvi; Lena Dixit; Krishnendu Roy

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
259 KB
Volume
79A
Category
Article
ISSN
1549-3296

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Thymocyte development takes place in a complex milieu of supportive cells and ECM that are responsible for the proliferation, adhesion, migration, and selection processes these cells undergo before reaching maturity. In recent years, the role of notch signaling in lymphocyte development, specifically T‐cell development, has been extensively characterized. Although notch ligand mediated signals have been shown to be a necessary component of T‐cell generation from stem cells, high‐throughput, synthetic biomaterial‐based systems for notch‐directed stem‐cell differentiation into lymphocytes are yet to be reported. Here, we present a microbead‐based, artificial notch signaling system to study stem‐cell differentiation into the T‐cell lineage. Magnetic microbeads were functionalized with the notch ligand DLL4 using streptavidin‐biotin binding and antibody–antigen coupling. Immunohistochemistry and flow cytometry analysis indicated ∼65% conjugation efficiency. Efficient notch signaling through these functionalized microbeads was demonstrated through a myotube inhibition assay in C2C12 myoblasts. Thy1.2^+^ early T cells were successfully generated from mouse bone marrow hematopoietic stem cells (BMHSCs) using DLL4 functionalized beads using both insert‐based and mixed stromal cell (OP9) coculture conditions, indicating that stem cell–stromal cell physical contact is not necessary for DLL4 directed T‐cell differentiation. Coculture studies with bead‐to‐cell ratios of 1:1 generated higher T‐cell differentiation efficiencies, compared to bead‐to‐cell ratios of 5:1. These data demonstrate the promising potential of this biomaterial‐based notch signaling system to generate T cells from stem cells and to elucidate the molecular interactions in T‐cell development. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

📜 SIMILAR VOLUMES

Biomaterial-based notch signaling for th
Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells
✍ Sabia Taqvi; Lena Dixit; Krishnendu Roy 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 54 KB

Thymocyte development takes place in a complex milieu of supportive cells and ECM that are responsible for the proliferation, adhesion, migration, and selection processes these cells undergo before reaching maturity. In recent years, the role of notch signaling in lymphocyte development, specificall

Mimicking cell–cell interactions at the
Mimicking cell–cell interactions at the biomaterial–cell interface for control of stem cell differentiation
✍ Benjamin L. Beckstead; Daniel M. Santosa; Cecilia M. Giachelli 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 383 KB

## Abstract The ability to regulate stem cell proliferation and differentiation has relevance in numerous medical applications, including medical devices, tissue engineering, and regenerative medicine. To control cellular behavior at the biomaterial or scaffold interface, many studies have employed

Altered T cell differentiation and Notch
Altered T cell differentiation and Notch signaling induced by the ectopic expression of keratin K10 in the epithelial cells of the thymus
✍ Mirentxu Santos; Paula Río; Sergio Ruiz; Jesús Martínez-Palacio; Carmen Segrelle 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 971 KB

Transgenic mice expressing hK10 under the keratin K5 promoter display several alterations in the epidermis including decreased cell proliferation, and reduced susceptibility to tumor development. Given that K5 promoter is also active in the epithelial cells of the thymus, we explored the possible al

Hematopoietic stem cell differentiation
Hematopoietic stem cell differentiation affects expression and function of MRP4 (ABCC4), a transport protein for signaling molecules and drugs
✍ Lena Oevermann; Jan Scheitz; Katharina Starke; Kathleen Köck; Thomas Kiefer; Got 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 French ⚖ 387 KB

## Abstract Several types of peripheral blood cells express ABC transporters. ABCC4 (MRP4) and ABCC5 (MRP5) localize to different cellular sites and fulfill lineage‐specific functions such as mediator storage in platelets' dense granules. All mature blood cells originate from the same precursor and