Biomass Conversion || Single-Molecule Tracking of Carbohydrate-Binding Modules on Cellulose Using Fluorescence Microscopy

Analysis of exposed cellulose surfaces i

Analysis of exposed cellulose surfaces in pretreated wood biomass using carbohydrate-binding module (CBM)–cyan fluorescent protein (CFP)

✍ Takeshi Kawakubo; Shuichi Karita; Yuko Araki; Shota Watanabe; Masafumi Oyadomari 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 817 KB

## Abstract In enzymatic saccharification of lignocellulosics, the access of the enzymes to exposed cellulose surfaces is a key initial step in triggering hydrolysis. However, knowledge of the structure–hydrolyzability relationship of the pretreated biomass is still limited. Here we used fluorescen