Biomarker discovery in rat plasma for estrogen receptor-α action

Trace LC/MS/MS quantitation of 17β-estra

Trace LC/MS/MS quantitation of 17β-estradiol as a biomarker for selective estrogen receptor modulator activity in the rat brain

✍ Chris Petucci; Tom Lloyd; Heather A. Harris; Xiaochun Zhang; Vargheese M. Chenna 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 142 KB

## Abstract A sensitive LC/MS/MS method has been developed by derivatization of 17β‐estradiol (E2) with dansyl chloride to quantitate 17β‐E2 in female rat serum. The use of E2‐d~5~ minimized interferences from endogenous 17β‐E2 in order to achieve a limit of quantitation (LOQ) of 2.5 pg/ml using 15