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Bioluminescent assay of femtomole levels of estrone and estradiol

✍ Scribed by J.C. Nicolas; A.M. Boussioux; A.M. Boularan; B. Descomps; A.Crastes de Paulet

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 354 KB
Volume: 135
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90742-x

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✦ Synopsis

A rapid and very sensitive enzymatic assay of estrogens was developed using the transhydrogenase reaction of the human estradiol-178 dehydrogenase and bioluminescence for the quantification of NADH accumulated during the first reaction. The assay requires two steps: first, the addition of transhydrogenase buffer, and a few minutes later the addition of the bioluminescence reagent. The method can determine 0.1 to 50 pg of estrogens in the assay, in 1 h, with a coefficient of variation of 5%.

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