Biological functionalization and fluorescent imaging of carbon nanotubes

✍ Scribed by Zuxun Xu; PingAn Hu; Shimin Wang; Xianbao Wang

• Book ID: 104002152
• Publisher: Elsevier Science
• Year: 2008
• Tongue: English
• Weight: 568 KB
• Volume: 254
• Category: Article
• ISSN: 0169-4332
• DOI: 10.1016/j.apsusc.2007.07.189

✦ Synopsis

Biofunctionalization and manipulating of carbon nanotubes (CNTs) is important for biomedical research and application. Cy5 labeled goat antirabbit IgG (anti-IgG-Cy5) is chemically bonded to CNTs via a two-step process of diimide-activated amidation. This process can avoid the intermolecular connection of proteins. Fluorescent imaging of CNTs in aqueous solution has been demonstrated using anti-IgG-Cy5 immobilized CNTs (IgG-CNTs) as the model. The biologically functionalized carbon nanotubes (f-CNTs) in solution have been observed successfully using fluorescence microscopy. The fluorescent image of highly oriented f-CNTs is obtained at first time. The influencing factors on fluorescent imaging including oxidation duration, background noise and reactant concentration are discussed.