Biological energy transformations in tissue homogenates

✍ Scribed by Potter, Van R.

• Book ID: 102877916
• Publisher: Wiley (John Wiley & Sons)
• Year: 1945
• Tongue: English
• Weight: 745 KB
• Volume: 26
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030260204

✦ Synopsis

Attempts to appraise fundamental physiological phenomena in terms of the modern concepts of biological energy transformations are at present limited mainly by the availability of methods for the measurement of the enzymatic components of the energy transforming mechanisms. We have attempted to devise practical analytical techniques which are based upon preparations of tissue referred to as homogenates (Potter, '44 ; '45 a). These studies do not deny the importance of structure in tissues ; indeed, they are designed to utilize minute amounts of protoplasm and thus to facilitate the study of tissue and cellular components, and thereby to correlate visible structure with enzymatic properties.

Energy-requiring reactions such as function and chemical synthesis are usually thought to require intact cellular structure, but many of these reactions have now been carried out in cell-free preparations and by applying the fundamental studies of others (Kalckar, '39 ; Belitser,