Biological effect of He-Ne laser: Investigations on functional and micromorphological alterations of cell membranes, in vitro

✍ Scribed by Tamara Kubasova; Zoltán Somosy; Péter Unk; András Kókai; Lajos Kovács

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 560 KB
• Volume: 4
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.1900040411

✦ Synopsis

A 1-J/cm2 dose of single laser-irradiation applied on primary human embryo fibroblast culture was not followed either by functional or micromorphological alterations of cell surfaces. This dose, however, applied four times with 24-hour intervals changed the functional conditions as well as surface charges of cell membranes. As detection methods, radioactive glucosamine uptake, lectin, and cationized ferritin-binding techniques were applied. At the same time, the scanning and transmission electron microscopy of laser-irradiated cells did not reveal any micromorphological or ultrastructural alterations. The effect of a 5-J/cm2 dose did not differ from that of 1 J/cm2. It is suggested that laser radiation-induced circumstances on cell surfaces can contribute to the strength of cell-to-cell contacts, i.e., to the stimulation of epithelization experienced in the clinical gynecologic practice.