Biological characterization of a tumour-growth-promoting factor from ascitic fluid of tumour-bearing mice
✍ Scribed by Ole Henriksen; Lloyd W. Law
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- French
- Weight
- 343 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Tumour‐growth‐promoting activity has been observed in ascitic fluids of mice with progressively growing SV40‐ and chemically induced tumours. Administration of ascitic fluid to animals together with a normally non‐tumorigenic dose of SV40‐transformed tissue culture cells (TU‐5) will cause these cells to grow progressively and eventually kill the animals. Also, the mKSA‐specific, immunologically non‐responsive state (the eclipse phase) of mice bearing large mKSA tumours can be maintained by administration of ascitic fluid or serum from tumour‐bearing animals after the tumours have been surgically removed. Normally, complete immunity to a secondary tumour cell challenge is re‐established approximately 10 days after removal of the primary tumour, but daily injections of ascitic fluid or serum will maintain eclipse so that a secondary challenge of tumour cells will grow progressively. The active component apparently acts non‐specifically, since ascitic fluid from an unrelated tumour also is able to maintain the mKSA‐specific eclipse phase. The ascitic fluid has no apparent immunosuppressive activity in vivo.