Biological activity of chitosan–sugar hybrids: specific interaction with lectin
✍ Scribed by Xuebing Li; Yohsuke Tushima; Minoru Morimoto; Hiroyuki Saimoto; Yoshiharu Okamoto; Saburo Minami; Yoshihiro Shigemasa
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 140 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1042-7147
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✦ Synopsis
The specific interactions between lectins and chitosan± sugar hybrids, the synthesized chitosan derivatives linking carbohydrate residue to the amino group of chitosan, were investigated. The specific bindings of chitosan-L-fucose (Fuc) hybrid with Ulex europaeus agglutinin I (UEA I, a lectin specific to L-Fuc), and chitosan-N-acetyl-D-glucosamine (D-GlcNAc) hybrid with Concanavalin A (Con A, a lectin specific to D- glucose, D-mannose and D-GlcNAc), were confirmed by a surface plasmon resonance technique. The microscopic observation of Pseudomonas aeruginosa, which was preincubated with the fluorescein isothiocyanate-labeled chitosan-L-Fuc hybrid, showed bacteria aggregation. The aggregation was thought to be resulted from the specific interaction of the L-Fuc residue of the hybrid with PA-II lectin on the surface of P. aeruginosa. The chitosan-L-Fuc hybrid inhibited P. aeruginosa growth more effectively in comparison with the other hybrids or unmodified chitosan. The enhancement of antimicrobial activity of chitosan-L-Fuc hybrid could be attributed to the specific binding between PA-II lectin of P. aeruginosa and L-Fuc residue of the L-Fuc hybrid.