Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems
β Scribed by Jeng-Jie Wang; Harold E. Swaisgood; Jason C. H. Shih
- Book ID
- 101725473
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 221 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0006-3592
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A composite plasmid (pAT2010) has been constructed in vitro from RSF2124 and Bacillus subtilis IFO3022 plasmid (pAT1060) by covalent joining of the two DNA molecules by means of Escherichia coli DNA ligase through the cohesive ends generated by restriction endonuclease RI (EcoRI) cleavage. The compo
DNA from Clostridium acetobutylicum ABKn8 was partially digested with Sau3A and the fragments obtained were inserted into the unique BamHI site of the cloning vector pHV33. The recombinant plasmids were used to transform Escherichia coli HB101 with selection for ampicillin resistance. A collection o