𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Bioimaging TOF-SIMS: High resolution 3D imaging of single cells

✍ Scribed by Håkan Nygren; Birgit Hagenhoff; Per Malmberg; Mikael Nilsson; Katrin Richter

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
389 KB
Volume
70
Category
Article
ISSN
1059-910X

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✦ Synopsis

Abstract

The distribution of phosphocholine ions (m/z 184, m/z 86), sodium ions, and potassium ions in thyroid tumor cells was analyzed by imaging TOF‐SIMS. Repeated sputtering with a C source and subsequent analysis with a Bi gun produced a series of 138 images that were stacked to make a 3D display of the chemistry of cells. Phosphocholine was seen in the plasma membrane (m/z 184) and intracellular membranes (m/z 86). The different fragmentation of the phospholipid probably reflects the chemical composition of membranes at these sites. High intensity of secondary ion signals of potassium was seen in membrane‐encompassed cellular compartments. The data indicate that potassium ions are compartmentalized in thyroid tumor cells. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.

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