Biohydrogen production from sugarcane bagasse hydrolysate by elephant dung: Effects of initial pH and substrate concentration
✍ Scribed by Arunsri Fangkum; Alissara Reungsang
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 460 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0360-3199
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✦ Synopsis
Pre-heated elephant dung was used as inoculum to produce hydrogen from sugarcane bagasse (SCB) hydrolysate. SCB was hydrolyzed by H 2 SO 4 or NaOH at various concentrations (0.25e5% volume) and reaction time of 60 min at 121 C, 1.5 kg/cm 2 in the autoclave.
The optimal condition for the pretreatment was obtained when SCB was hydrolyzed by H 2 SO 4 at 1% volume which yielded 11.28 g/L of total sugar (1.46 g glucose/L; 9.10 g xylose/L; 0.72 g arabinose/L). The maximum hydrogen yield of 0.84 mol H 2 /mol total sugar and the hydrogen production rate of 109.55 mL H 2 /L day were obtained at the initial pH 6.5 and initial total sugar concentration 10 g/L. Hydrogen-producing bacterium (Clostridium pasteurianum) and non hydrogen-producing bacterium (Flavobacterium sp.) were dominating species in the elephant dung and in hydrogen fermentation broth. Sporolactobacillus sp. was found to be responsible for a low hydrogen yield obtained.