๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Biofluorescent substances for rapid assessment of toxicants on invertebrates

โœ Scribed by Leweke, Eva C. ;Kubitz, Jody A. ;Besser, John M. ;Giesy, John P.

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
526 KB
Volume
11
Category
Article
ISSN
1053-4725

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โœฆ Synopsis

The recently developed 1 h fluorescent test with Daphnia magna was further evaluated. The assay is based on the in vivo observation of enzymatic activity using a fluorogenic substrate that, after cleavage by the corresponding enzyme, releases a fluorescent dye. Influences interfering with the test evaluation were determined and eliminated.

It was investigated as to whether new fluorescent tests can be developed for Ceriodaphnia dubia and Hyalella azteca. Also, other fluorescent substrates were tested for their applicability as fluorogenic substrate in combination with D. magna, C. dubia, and H. azteca.

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