Bioelicitation of secondary metabolites in in vitro cultures of Gymnema sylvestre R.Br.

✍ Scribed by C. Subathra Devi; V. Mohana Srinivasan

• Book ID: 104064661
• Publisher: Elsevier
• Year: 2009
• Tongue: English
• Weight: 70 KB
• Volume: 25
• Category: Article
• ISSN: 1871-6784
• DOI: 10.1016/j.nbt.2009.06.643

✦ Synopsis

Aspergillus niger cell extract was used as a elicitor. Growth rate and the gymnemic acid production of the elicited and non-elicited cultures of Gymnema sylvestre were investigated. The growth rate of the elicited and non-elicited cultures was not significantly different, but the gymnemic acid yield was very high in elicited cultures. When compared to non-elicited cultures, 8-9-fold increase of gymnemic acids yield in elicited cultures was noticed. 10 g of cell suspension produced in shake flask culture was transferred to 5 l batch fermentor containing 2-l MS medium. Fresh weight was increased 6.5 times after 20 days of culture and the gymnemic acid production achieved was 900 mg/l. After elicitation, 8 times increase of fresh weight was gained. When compared to shake flask culture, 8-9-fold increase in gymnemic acid production was noticed in fermentors.