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Bioelectrochemical Response and Kinetics of Choline Oxidase Entrapped in Polyaniline-Polyacrylonitrile Composite Film

✍ Scribed by Huai-Guo Xue; Zhi-Quan Shen


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
332 KB
Volume
20
Category
Article
ISSN
0256-7660

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✦ Synopsis


Abstract

A novel choline oxidase electrode was constructed by entrapping choline oxidase into polyaniline‐polyacrylonitrile composite film. The enzyme film was prepared by in situ electropolymerization of aniline into porous polyacrylonitrile‐coated platinum electrode in the presence of choline oxidase. The enzyme electrode exhibited sensitive and stable electrochemical response to choline. The kinetics analysis showed that the mass transport is partially rate‐limiting. The influences of pH, applied potential and temperature on the response of the enzyme electrode were also described.