Bioelectrocatalytic Oxidation of Glucose by Immobilized Bacteria Gluconobacter oxydans. Evaluation of Water-Insoluble Mediator Efficiency
✍ Scribed by Elena Babkina; Ekaterina Chigrinova; Ol'ga Ponamoreva; Valery Alferov; Anatoly Reshetilov
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 106 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Abstract
For the first time it has been shown possible to use strain G. oxydans sbsp. industrius VKM B‐1280 in combination with water‐insoluble mediators: ferrocene, 1,1′‐dimethylferrocene, 2,5‐dibromo‐1,4‐benzoquinone, and 2‐methyl‐1,4‐benzoquinone in the process of electrocatalytic oxidation of glucose. Cell‐mediator interaction has been studied using a carbon paste electrode that comprised a mediator and immobilized bacteria. Catalytic activity of the system “mediator – bacterial cell – substrate” was characterized by three quantities: the maximum reaction rate and the ratios of the Michaelis constant to the distribution constant for the substrate and to that for the electron acceptor. It has been revealed that 2,5‐dibromo‐1,4‐benzoquinone is the most efficient for high catalytic activity.