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Biodiesel production from Stichococcus strains at laboratory scale

✍ Scribed by Giuseppe Olivieri; Antonio Marzocchella; Roberto Andreozzi; Gabriele Pinto; Antonino Pollio

Publisher: Wiley (John Wiley & Sons)
Year: 2011
Tongue: English
Weight: 239 KB
Volume: 86
Category: Article
ISSN: 0268-2575
DOI: 10.1002/jctb.2586

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✦ Synopsis

BACKGROUND: This paper reports the results of an experimental campaign of autotrophic cultures of Stichococcus strains aiming at selecting the most promising strain for biofuel production. The strain selected -S. bacillaris 158/11 -was cultivated in 1 L lab-scale bubble column photobioreactors under fed-batch and semi-continuous conditions. A Bold basal medium supplemented with NaNO 3 as nitrogen source was adopted. Tests were carried out at 23 • C, 140 µE m -2 s -1 , and air flow rate ranging between 0.4 and 4 vvm. Cultures were characterized in terms of pH, concentration of total nitrogen, total organic carbon, total inorganic carbon, biomass, lipid fraction and methyl-ester distribution of transesterified lipids. RESULTS: S. bacillaris 158/11 proved to be the best strain to produce biodiesel. Methyl-ester distribution was characterized by a large fraction of methyl palmitate, methyl linolenate, methyl linoleate, and methyl oleate along with phytol. The process photosynthetic efficiency -fraction of available light stored as chemical energy -was about 1.5%. Specific biomass productivity was ∼60 mg DM L -1 day -1 under the semi-continuous conditions tested. Total lipid productivity was 14 mg L -1 day -1 at a dilution rate of 0.050 L day -1 . CONCLUSION: S. bacillaris 158/11 is a potential strain for massive microalgae cultures for biofuel production. Higher biomass/total-lipid productivity could be obtained in sunlight.

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