Biodegradation of a poly(ester)urea-urethane by cholesterol esterase: Isolation and identification of principal biodegradation products

✍ Scribed by Wang, G. B. ;Labow, R. S. ;Santerre, J. P.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 305 KB
• Volume: 36
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19970905)36:3<407::aid-jbm16>3.0.co;2-a

✦ Synopsis

Synthesized poly(ester)urea-urethanes with 14 C-trometry. Both products are TDA derivatives (secondary arolabeled toluene diisocyanate or 14 C-labeled chain extender matic diamine) substituted with end units of the polyester ethylene diamine were incubated with cholesterol esterase segment at N and NЈ positions of TDA. The absence of free in a phosphate buffer solution at 37ЊC. A number of biodegra-TDA suggests that there could be a stabilization of urethane dation products, generated at the level of 2.8 g/cm 2 of and urea linkages within the toluene diisocyanate (TDI) segpolymer surface area, were isolated from this simulated ments of the polyurethanes. For TDI-synthesized polymers, physiologic system. Individual products were obtained by this finding raises awareness to the potential biological imseparation with reversed-phase high-performance liquid portance of degradation products other than TDA, particuchromatography. The two different radiolabels were used to larly to their interaction with surrounding cells. © 1997 John assist in the identification of degradation products from hard-Wiley & Sons, Inc. J Biomed Mater Res, 36, 407-417, 1997. and soft-segment domains. Approximately 20 degradation products were isolated; however, toluene diamine (TDA) was not detected from the chromatographic separation. Two