Biodegradable honeycomb collagen scaffold for dermal tissue engineering

✍ Scribed by Joseph George; Jun Onodera; Teruo Miyata

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 321 KB
• Volume: 87A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32277

✦ Synopsis

Abstract

Tissue engineering requires a mechanically stable, biocompatible, and biodegradable scaffold that permits cell adherence and proliferation, allows preservation of cell‐specific properties, and suitable for surgical implantations. In this study, honeycomb collagen sheet was used for three‐dimensional (3D) cultures of human skin fibroblasts and characterized as an effective and suitable scaffold for dermal tissue engineering. About 1‐mm‐thick honeycomb collagen sheets, prepared from bovine dermal atelocollagen, cross‐linked by UV‐irradiation, and sterilized by heat, were placed on the proliferating fibroblasts on day 3 of the culture. The cells attached quickly to the collagen scaffold, proliferated inside the honeycomb pores, and formed a structure similar to dermis within 60 days. On day 60, total cellular DNA content of the 3D cultures was 12‐fold higher when compared with the 2D control cultures without the scaffold. Measurement of procollagen type I in the media demonstrated a 20‐fold increase. Scanning electron microscopy of the 3D cultures showed a well‐formed structure similar to dermis and biodegradation of the honeycomb collagen scaffold. Our study proved that honeycomb collagen sheet is a mechanically stable, biocompatible and biodegradable scaffold for dermal tissue engineering, and also potentially useful for other cell‐based therapies and tissue engineering applications. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008