Bioconcentration of triphenyltin (TPT) and tributyltin (TBT) was studied in the freshwater organisms Daphnia magna (zooplankton), Chironomus riparius (sediment organism) and Thymallus thymallus (fish yolk-sac larvae). TPT bioconcentration factors (BCFs) at pH 8 were highest for Thymallus (2200), followed by Chironomus (680) and Daphnia (190). The differences could not be fully explained by different total lipid contents. Metabolism and lower bioconcentration were observed for TBT in Chironomus. The BCFs of both TBT and TPT were higher at pH 8 than at pH 5, but the difference was much less pronounced than predicted by the octanol-water partition model. This suggests that, besides the hydroxide species (TBTOH and TPTOH), the cations (TBT and TPT ) are also taken up by the organisms to some extent and that the octanol-water partition model underestimates the uptake of the charged species. Low concentrations of humic substances (HS) led to small reduction in the bioconcentration of TPT in Daphnia and Thymallus, and a significant reduction occurred at relatively high concentrations of HS (b10 mg C l Γ1 ). The results of this study provide an important basis for future investigations aiming at a better understanding of the bioavailability and fate of TBT and TPT in freshwater ecosystems.