Biocompatibility test procedures for materials evaluationin vitro. I. Comparative test system sensitivity
✍ Scribed by Johnson, H. J. ;Northup, S. J. ;Seagraves, P. A. ;Garvin, P. J. ;Wallin, R. F.
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 838 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Relative sensitivity of in vitro biocompatibility test systems was explored. Cellular responses of 12 standardized cell lines to 20 materials representing a range of toxicity were measured. Each cell line and material combination was tested in duplicate using four different culture methods, and each culture plate was examined by two graders. Results of the tissue culture assays were compared to those obtained for the same materials ~n vivo using a 5-day rabbit intramuscular implant assay. Methods involving measurement of cellular growth (colony counts or percent of confluence) in serumfortified media extracts of test samples were generally more sensitive and discriminating than those in which test materials were placed directly in cell cultures (measurement of zone of growth inhibition). .There was good agreement between graders for all test methods. Antibiotics were not used in the test program. Based upon sensitivity, reproducibility, ability to discrimindte materials, and grader agreement, 4 of the 12 cell lines and 2 of the 4 test methods appeared most suitable for screening and evaluation of materials. Agreement of results using these four cell lines with intramuscular implantation tests for the 30 materials ranged from 60 to 90%. different cultures varied from similar14-18 to noticeably different.I0J3J9 In comparison to in uiuo methods, cell culture techniques have been variously reported as being p r e d i c t i ~e , ~, ~~, ~~ and more ~e n s i t i v e . ~J ~ However, the procedures described in these studies were generally limited to one or a very few cell cultures in combination with one or perhaps two test methods. This study explored the premises that cell cultures derived from different tissues and species vary significantly in their sensitivity to environmental changes and that cell culture test methods differ appreciably in accuracy. Both in uitro and concurrent in uivo test data were analyzed to determine which cell Present address Standard 0 1 1 Co (Indiana), Chicago, Illlnols 60601