Biochemical toxicology of argemone oil. I. effect on hepatic cytochrome P-450 and xenobiotic metabolizing enzymes

✍ Scribed by Kaushal K. Upreti; Mukul Das; Subhash K. Khanna

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 598 KB
• Volume: 11
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.2550110309

✦ Synopsis

The in vivo effect of argemone oil on hepatic xenobiotic metabolizing enzymes was investigated in albino rats following either a single (10 ml kg-' body wt.) or multiple intraparenteral doses (5 ml kg-' body wt.) for three days. Animals sacrificed 72 h after a single intraparenteral dose of argemone oil exhibited a significant 105s of hepatic cytochrome P-450 (35%) and cytochrome b5 (34%) contents and inhibition of aminopyrine-Ndemethylase (APD), aryl hydrocarbon hydroxylase (AHH) and ethoxycoumarin-Odeethylase (ECD) activities (2139%). Three successive 24-hourly intraparenteral injections of argemone oil followed by sacrificing the animals after 24 h of the last injection, showed a greater degree of inhibition of the content of cytochrome P-450 (58%) and its dependent mixed-function oxidases (3543%). Also, multiple treatment of argemone oil caused a depletion of endogenous hepatic glutathione (GSH) content (72%) with a concomitant increase in lipid peroxidation (177%) and decrease in glutathione-S-transferase (GST) activity (30%). A Significant decrease in relative liver weight (39%) was observed in animals treated with multiple treatment of argemone oil. These results suggest that argemone oil can alter both membrane and cytosolic defences and destabilizes the hepatic cytochrome P-450 dependent mixed-function oxidase system, so that it tips in the direction of autooxidative peroxidation of lipids.