Biochemical evidence of a translocation between6 RL/7 RL chromosome arms in rye (Secale cerealeL.). A genetic map of6Rchromosome
✍ Scribed by C. Benito; F. J. Gallego; C. Zaragoza; J. M. Frade; A. M. Figueiras
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 796 KB
- Volume
- 82
- Category
- Article
- ISSN
- 0040-5752
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✦ Synopsis
The segregation of different isozymic loci was investigated in backcrosses and F2s in rye. The leucin aminopeptidase-1 (Lap-1), Aconitase-1 (Aco-1), Esterase-6 (Est-6), Esterase-8 (Est-8), and Endopeptidase-1 (Ep-1) loci were linked. The Aco-1, Est-6, and Est-8 loci have been previously located on the 6RL chromosome arm. The Lap-1 locus has been located on the 6RS chromosome arm. The results favor the gene order: Lap-1... (centromere)... Aco-1... Est-8... Est-6... Ep-1. The isoelectric focusing separations of aqueous extracts from mature embryo tissue of wheat-rye addition and substitution lines involving the chromosomes of cereal rye Secale cereale L. confirmed the gene location of locus Ep-1 on the 6RL chromosome arm. Screening of wheat-rye addition lines involving the chromosomes of Secale montanum revealed that Ep-1 locus is not located on chromosome 6R of S. montanum. These results are the first biochemical evidence of the translocation between chromosome arms 6RL/7RL in the evolution of S. cereale from S. montanum.