Biochemical Evidence for the Bidirectional Replication of DNA in Escherichia coli
โ Scribed by McKENNA, W. G.; MASTERS, MILLICENT
- Book ID
- 109684436
- Publisher
- Nature Publishing Group
- Year
- 1972
- Tongue
- English
- Weight
- 392 KB
- Volume
- 240
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/240536a0
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When E. coli F+ cells carrying the dna-167 or dnaC2 mutation, which causes the temperature-sensitive initiation of DNA replication, are exposed to a non-permissive temperature to stop the replication of chromosome and F factor, and then transferred back to a permissive temperature with the addition
Temperature-sensitive mutants defective in the initiation of DNA replication are exposed to a non-permissive temperature to complete already initiated replication, and are transferred back to a permissive temperature. DNA synthesis can resume in the presence of rifampicin or rifampicin plus chloramp
F' strains of E. coli have been isolated which are merodiploid for various chromosomal segments between 66 and 78 minutes. Strains diploid for the chromosomal DNA between the genes bgl and mtl grow slowly, have a reduced DNA/mass and an increased cell size. These properties could result if the chrom