Biochemical characterization of φX174-protein-E-mediated lysis of Escherichia coli
✍ Scribed by Angela WITTE; Werner LUBITZ
- Book ID
- 115126213
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 895 KB
- Volume
- 180
- Category
- Article
- ISSN
- 1432-1327
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The largest of the fragments produced by AluI digestion of phi X174 RFI DNA comprises genes E and J as well as parts of genes D and F. This DNA fragment (1007 bp) was cloned into the lac z' gene of plasmid pUR222. In the recombinant plasmid pUH12, transcription of the phi X174 genes is controlled by
Expression of cloned gene E of bacteriophage PhiX174 induces lysis by formation of a transmembrane tunnel structure in the cell envelope of Escherichia coli. Ultrastructural studies of the location of the lysis tunnel indicate that it is preferentially located at the septum or at polar regions of th