Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain ofPasteurella multocida

✍ Scribed by R. S. Conrad; C. Galanos; F. R. Champlin

• Book ID: 104742677
• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 799 KB
• Volume: 20
• Category: Article
• ISSN: 0165-7380
• DOI: 10.1007/bf00366917

✦ Synopsis

Lipopolysaccharides were extracted from freeze-dried cells of Pasteurella multocida strain P-1581 (serotype 8) by the phenol-chloroform-petroleum ether method and biochemically analysed using standard procedures. The primary neutral sugars were glucose, galactose and heptose. No deoxy sugars were detected. Amino sugars included galactosamine, glucosamine and glucosamine-6-phosphate. 3-Deoxy-D-manno-2-octulosonic acid was present at a relatively low concentration. The analyses included identification and quantification of phosphate and alanine. The primary fatty acids and their approximate relative ratios were 3-hydroxytetradecanoate and tetradecanoate 2:1. Tetradecanoic acid was bound almost exclusively by ester linkages. 3-Hydroxytetradecanoic acid was bound primarily by amide linkages, although significant numbers of ester-bound residues were noted. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses indicated that the lipopolysaccharides were of low molecular weight.