✍ Scribed by M. Schülein; J. L. Daehnfeldt; P. Briand
No coin nor oath required. For personal study only.
Discontinuation and recommencement of estrone‐progesterone treatment causes regression and regrowth, respectively, of transplantable hormonedependent GR mouse mammary tumors. This tumor model was found convenient for the demonstration of hormonal responses in hormone‐dependent mouse mammary tumor cells. Tumor regression was palpable 2 days after discontinuation of hormonal treatment and tumors reached half their size within 3‐6 days. At this point, hormones were readministered and 2‐4 days later the tumors had grown to their preregression size. In the growing and regressing tumor we have investigated: (1) the content of RNA, DNA and protein per g wet weight; (2) in vivo incorporation for 45 min of ^32^P‐orthophosphate into RNA and DNA and the ^35^S‐methionine incorporation into protein; (3) SDS polyacrylamide gel electrophoresis of soluble proteins after in vivo incorporation of ^14^C‐ and ^3^H‐leucine. The RNA content per g wet weight was found to decrease during regression and to increase during regrowth. DNA and protein content showed no variation. RNA/DNA ratio thus varied in parallel to the RNA content. The precursor incorporation into all three macromolecular species decreased during regression. The incorporation into DNA showed the most pronounced decrease. After readministration of hormones, regrowth was accompanied by a rapid increase in precursor incorporation into RNA, while the incorporation into DNA showed a lag of 1 to 2 days. SDS polyacrylamide gel electrophoresis was carried out with soluble proteins labelled in vivo with ^14^C‐ and ^3^H‐leucine during regression and regrowth, respectively. No differences in 14C/3H ratios could be demonstrated for the major fractions detectable in the electropherogram.
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## Abstract We have previously shown that hormone‐dependent (HD) but not hormone‐independent (HI) mammary tumors of GR mice concentrate iodide. Since hormone dependence is lost during serial transplantation of the tumors, we have investigated whether the ability to concentrate iodide is lost simult