Biochemical and physical studies of the role of intrinsic metal ions in RNA polymerase from Escherichia Coli

Physical mapping of a collection of MaeI

Physical mapping of a collection of MaeI-generating amber mutations in the β gene of Escherichia coli RNA polymerase and the functional effect of internal deletions constructed through their manipulation

✍ Nihal Buyukuslu; Susan M. Trigwell; Ping-Ping Lim; Nobuyuki Fujita; Akira Ishih 📂 Article 📅 1997 🏛 Blackwell Publishing 🌐 English ⚖ 454 KB

We report the fine mapping of 55 of our 95 amber mutations in the β gene of __Escherichia coli__ RNA polymerase by virtue of the unique __Mae__I restriction sites created by this subset of nonsense mutations (i.e. CTAG, where the amber codon is underlined). [The full data are reported in Supplementa