Biochemical and morphological analysis of dentin following selective caries removal with a fluorescence-controlled Er:YAG laser
✍ Scribed by Søren Jepsen; Yahya Açil; Thorsten Peschel; Konstantinos Kargas; Jörg Eberhard
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 383 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0196-8092
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✦ Synopsis
Abstract
Background and Objective
We evaluated the endpoint of caries removal with a novel fluorescence‐controlled Er:YAG laser by biochemical analysis of dentin collagen and electron microscopy.
Materials and Methods
For the biochemical analysis 210 carious and 60 caries‐free teeth were treated by a fluoresence controlled Er:YAG laser (n = 240) or by conventional drilling (n = 30). A layer of dentin from the cavity floor was removed to determine collagen crosslinks hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) by HPLC. Thirty teeth were examined by SEM and TEM.
Results
Approximately 100% HP and LP were found in carious dentin, in contrast to 0.33% (HP) and 0.68% (LP) in sound dentin. Following caries removal by laser 0.84% (HP) and 1.26% (LP) and 1.56% (HP) and 2.48% (LP) (threshold level 5, respectively 10) were found.
Conclusion
The biochemical and ultrastructural analysis demonstrated the suitability of the fluorescence controlled Er:YAG laser to remove irreversibly denatured collagen of carious dentin. Lesers Surg. Med. 40:350–357, 2008. © 2008 Wiley‐Liss, Inc.